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    1. Our service

      Centre Testing International Co., Ltd. (CTI) is the pioneer and leader in the TIC Industry which provides one-stop solutions on testing, inspection, certification, calibration, audit, training & technical services.

       

      By Industry

      Our service capabilties cover the upstream and downstream of the supply chain including textile and apparel,toys,electronic appliances,medical health,food...andother industries.

      Specialty

      Comprehensively guarantee quality and safety, promote compliance and innovation, demonstrate brand competitiveness, and achieve higher quality, healthier, safer, and greener sustainable development.

      AUTHORITY & TRUST
      QUALITY & VALUE
      Composition Analysis of Cell Culture Media/Fluids

      The main components of cell culture medium/fluid include inorganic salts, trace elements, amino acids, vitamins, organic acids, nucleosides, purines, etc. CTI Sinotest provides you with quantitative analysis of 30 trace elements and 60 organic compounds, and semi-quantitative scanning analysis of more than 60 elements and 100 organic compounds.

      Composition Analysis of Cell Culture Media/Fluids

      Service Background

      The composition of cell culture medium/fluid is critical to cell growth. Monitoring changes in key components of the culture medium is an important part of the biopharmaceutical process.

       

      Applicable Products

      Applicable to synthetic cell culture media/fluids.
       

      Service Content

       

       

      Semi-quantitative scanning analysis

      Semi-quantitative scanning of elements in culture media by ICP-MS to quickly determine the types and range of trace elements. Semi-quantitative scanning analysis of organic matter in culture medium by LC-MSMS method, choosing analogues as internal standards.

       

       

      Quantitative analysis

      ICP-OES was used to quantitatively analyze the inorganic salts with high content in the medium; ICP-MS was used to quantitatively analyze the trace elements with low content in the medium; and LC-QQQ was used to quantitatively analyze the target substances. The methodological validation meets the relevant requirements of ICH Q2 R2 guidelines.

       

      Featured Services

      Detection of about 30 kinds of inorganic salts and trace elements.

      Category

      Serial number

      Element symbol

      Testing instrument

      Inorganic Salts

      1

      Na

      ICP-OES/MS

      2

      K

      ICP-OES/MS

      3

      Ca

      ICP-OES/MS

      4

      Mg

      ICP-OES/MS

      5

      P

      ICP-OES/MS

      6

      S

      ICP-OES/MS

      Trace Elements

      1

      Fe

      ICP-OES/MS

      2

      Co

      ICP- MS

      3

      Ni

      ICP- MS

      4

      Cu

      ICP- MS

      5

      Zn

      ICP- MS

      6

      Se

      ICP- MS

      7

      Cr

      ICP- MS

      8

      Mn

      ICP- MS

      9

      Mo

      ICP- MS

      10

      B

      ICP- MS

      11

      Li

      ICP- MS

      12

      Al

      ICP- MS

      13

      Si

      ICP- MS

      14

      V

      ICP- MS

      15

      Sn

      ICP- MS

      16

      Ge

      ICP- MS

      17

      Sr

      ICP- MS

      18

      Rb

      ICP- MS

      19

      Ba

      ICP- MS

      20

      Zr

      ICP- MS

      21

      Pb

      ICP- MS

      22

      Ag

      ICP- MS

      23

      Ti

      ICP- MS

      24

      Cd

      ICP- MS

      25

      As

      ICP- MS

       

      Detects 24 amino acids, including 10 essential amino acids and 14 non-essential amino acids for cell growth.

      Classification

      No.

      Name

      Abbreviation

      Essential Amino Acids

      1

      L-Isoleucine

      Iso

      2

      L-Leucine

      Leu

      3

      L-Lysine

      Lys

      4

      L-Methionine

      Met

      5

      L-Phenylalanine

      Phe

      6

      L-Threonine

      Phe

      7

      L-Tryptophan

      Try

      8

      L-Valine

      Val

      9

      L-Histidine

      His

      10

      L-Glutamine

      Gln

      Nonessential Amino Acids

      1

      L-Alanine

      Ala

      2

      L-Arginine

      Arg

      3

      L-Aspartic acid

      Asp

      4

      L-Cystine

      Cys-cys

      5

      L-Glutamic acid

      Glu

      6

      Glycinev

      Gly

      7

      L-Proline

      Pro

      8

      L-Serine

      Ser

      9

      L-Tyrosine

      Tyr

      10

      L-Cysteine

      Cys

      11

      L-Asparagine

      Asn

      12

      Taurine

      Tau

      13

      L-Ornithine

      Orn

      14

      Hydroxy-L-proline

      HyPro

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